Vortragstitel: Spatiotemporal control in synthetic and living cells using light
Bottom-up synthetic biology aims to construct cell-like systems starting from molecular building blocks and give insight into principles that give rise to cell function. Many functions in cells arise directly from the spatial and temporal control of processes such as protein localization, cell migration, tissue assembly and cell-to-cell communication. In this talk, I will present strategies of how such spatiotemporal control over adhesions in synthetic cells can be achieved with visible light using photoswitchable proteins and functions that arise from these. The photoswitchable adhesions allow us to recapitulate cell migration, to self-assemble and self-sort synthetic cells into multicellular functional architectures with high precision. Moreover, the organization in these multicellular communities is of significance for their communication and the overall arising behaviors. These synthetic cell-mimetic systems, which reduce complexity and yet capture key features of natural cells, allow us to quantify and correlate cell behavior with molecular information. Further, complementary approaches pursued with synthetic minimal cells as well as bacterial and mammalian cells allow translating concepts between different systems and integration into hybrid structures. Overall, our work on one hand provides insight into underlying design principles of life and on the other hand engineer new synthetic cell biology.